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Invitrogen™ TGFb1/LAP Human Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of LAP. TGF beta is synthesized as a precursor that contains LAP at the N-terminus and mature TGF beta at the C-terminus

Marque:  Invitrogen™ 88-50390-22

Informations supplémentaires : Poids : 0.10000kg

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Code produit. 15268897

  • 205.00€ / 2 kits


Date dexpédition estimée
du fournisseur 23-12-2019
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Includes: Capture Antibody Concentrate
Detection Antibody Concentrate
Human LAP standard (lyophilized): 20ng/mL upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent B
pre-titrated Streptavidin-HRP
Tetramethylbenzidine (TMB) Substrate Solution

Description

Description

TGF beta-1 (TGFB1, Transforming Growth Factor Beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines, found almost ubiquitously in tissues. Transforming growth factor (TGF)-b is stored in the extracellular matrix as a latent complex with its pro-domain. Activation of TGF beta-1 requires the binding of aV integrin to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-b binding proteins. Latent forms are complexes of TGF beta-1, an amino-terminal portion of the TGF-beta precursor, designated TGF-LAP (TGF-latency associated peptide), and a specific binding protein, known as LTBP. TGF beta-1 helps regulates proliferation, differentiation, adhesion, migration in many cell types. Many cells have TGF beta receptors, and the protein positively and negatively regulates many other growth factors. TGF beta-1 is cleaved into a latency-associated peptide and a mature TGF beta-1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGF beta-1 homodimer. The mature peptide may also form heterodimers with other TGF beta family members. The gene for TGF beta-1 is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease and cystic fibrosis.
Spécification

Spécification

P01137
Supernatant
156.3-10,000 pg/mL
ELISA
Colorimetric Microplate Reader
ELISA Kit
156.3 pg/mL
Plasma, 10 μL|Serum, 10 μL|Supernatant, 10 μL
1 hr. 45 min.
Human
Latency Associated Peptide, Pro-TGF beta 1, LAP/TGF beta 1
2 x 96 Tests
Human
Biotin
7040
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
RUO
10 μL
2°C to 8°C
24 hr. 30 min.
TGFB1
HRP
Vidéos
Documents

For Research Use Only