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Proteintech™ Human/Mouse/Rat GDF-8 ELISA Kit

Product Code. 16881526 Shop All Proteintech Products
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Target Species:
Human, Mouse, Rat
Unit Size:
96 tests
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Product Code. 16881526

Brand: Proteintech™ KE0012096T

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This item is not returnable. View return policy

Description

KE00120 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-8 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-8.

  • Growth Differentiation Factor 8 (GDF-8), also called myostatin, is a member of the transforming growth factor (TGF)-βsuper-family. GDF-8 is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein.
  • GDF-8 is also expressed in the human reproductive system, such as in granulosa cells, follicular fluid and trophoblasts. In addition, GDF-8 has also functions in heart and adipose tissue, and is related to interstitial fibrosis in the heart, human cancer cachexia, and activation of inflammatory cytokines and insulin resistance.
  • The assay recognizes human,mouse and Rat GDF-8. An antibody specific for GDF-8 has been pre-coated onto the microwells. The GDF-8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GDF-8 is added to detect the captured GDF-8 protein.
  • For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Specifications

Accession Number BC074757
Assay Range 15.6 to 1000 pg/mL, 31.25 to 2000 pg/mL
Assay Sensitivity 2.3 pg/mL
Certifications/Compliance ISO 13485
Detection Method Sandwich ELISA, Double Antibody
Immunoassay Kit Format Coated ELISA Kit
Sample Type Plasma, Serum
Substrate DAB
Target Species Human, Mouse, Rat
Gene ID (Entrez) 2660
Host Species Human/Mouse/Rat
Immunoassay Kit Grade Solid
Interassay CV <10%
Intraassay CV <10%
Shipping Condition Blue ice
Storage Requirements -20°C to +4°C
Total Assay Time 4.5 hr.
UniProt ID O14793(Human)/O08689(Mouse)/O35312(Rat)
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Safety and Handling

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Product Identifier
  • 96TEST Human/Mouse/Rat GDF-8 ELISA Kit
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 1
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H318-Causes serious eye damage.
  • H319-Causes serious eye irritation.
  • H332-Harmful if inhaled.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P201-Obtain special instructions before use.
  • P202-Do not handle until all safety precautions have been read and understood.
  • P234-Keep only in original container.
  • P264-Wash skin thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P330-Rinse mouth.
  • P362-Take off contaminated clothing.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : Sulphuric Acid, Tetramethylbenzidine
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