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  5. Invitrogen™ Human sCD134/OX40 (Soluble) ELISA Kit

Invitrogen™ Human sCD134/OX40 (Soluble) ELISA Kit

Product Code. 15358120
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
960 tests
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Product Code. 15358120

Brand: Invitrogen™ BMS296TEN

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD134/OX40
Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
Streptavidin-HRP
Human sCD134/OX40 Standard lyophilized, 10ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human soluble CD134/OX40 ELISA quantitates Hu CD134/OX40 in human serum, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD134/OX40. Principle of the method The Human CD134/OX40 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P43489
Assay Range 78-5000 pg/mL
Assay Sensitivity 1.8 pg/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human sCD134/OX40
Biotin-Conjugate anti-human sCD134/OX40 monoclonal antibody
Streptavidin-HRP
Human sCD134/OX40 Standard lyophilized, 10 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; Ly-70; lymphoid activation antigene ACT35; MRC OX40; Ox40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 receptor; TNF receptor superfamily member 4; Tnfrsf4; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; tumor necrosis factor superfamily, member 4; Txgp1; Txgp1lACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; Ly-70; lymphoid activation antigene ACT35; MRC OX40; Ox40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 receptor; TNF receptor superfamily member 4; Tnfrsf4; tumor necrosis factor (ligand) superfamily member 4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; tumor necrosis factor superfamily, member 4; Txgp1; Txgp1l; ACT35; CD134; IMD16; OX40; TXGP1L
Gene ID (Entrez) 7293
Gene Symbol Tnfrsf4
Interassay CV 12%
Intraassay CV 8%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Lysis Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 25 μL, Supernatant 25 μL
Storage Requirements 2-8°C
Synonym OX 40 L receptor, ACT 35
Target OX40 (CD134)
Test Time 1 hr 20 min
Total Assay Time 3 hr 10 min
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