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  5. Invitrogen™ Human CD137 (4-1BB) (Soluble) ELISA Kit

Invitrogen™ Human CD137 (4-1BB) (Soluble) ELISA Kit

Product Code. 15501897
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Quantity:
10 x 96 Tests
96 Tests
Unit Size:
96 tests
Pack of 10
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Product Code. 15501897

Brand: Invitrogen™ BMS289TEN

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Description

Includes

Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD137
Biotin-Conjugate anti-human sCD137 polyclonal antibody
Streptavidin-HRP
Human sCD137 Standard lyophilized, 100ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films

ELISA

The Human Soluble CD137 (4-1BB) (Hu CD137) ELISA quantitates Hu CD137 in human serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD137. Principle of the method The Human CD137 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells. Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells. CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development. Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival. Soluble forms of CD137 may provide negative control mechanism for some immune responses.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q07011
Assay Range 0.78-50 ng/mL
Assay Sensitivity 0.2 ng/mL
Content And Storage Aluminum pouch(es) with a Microplate coated with monoclonal antibody to human sCD137
Biotin-Conjugate anti-human sCD137 polyclonal antibody
Streptavidin-HRP
Human sCD137 Standard lyophilized, 100 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
Conjugate HRP
Immunoassay Kit Format Sandwich ELISA Kit
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias 4 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 94 1 BB; 41BB; 4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; EGK_00183; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; Ly63; receptor protein 4-1BB; s4 1 BB; s4 1BB; s41BB; sCD137; secreted CD137 antigen; soluble 4 1 BB; soluble 4 1BB; soluble 41BB; soluble CD137; T cell antigen ILA; T-cell antigen 4-1BB; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9; 4-1BB; CD137; CDw137; ILA
Gene ID (Entrez) 3604
Gene Symbol TNFRSF9
Interassay CV 9.9%
Intraassay CV 7.1%
Kit Contents Pre-coated 96 well plate, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Sample Diluent, Assay Buffer concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye Biotin
Protein Aliases 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 50 μL, Supernatant 50 μL
Storage Requirements 2-8°C
Synonym 4-1 BB Ligand Receptor, TNFRSF9, ILA
Target 4-1BB (CD137)
Test Time 1 hr 20 min
Total Assay Time 3 hr 10 min
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