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Invitrogen™ Human Troponin I ProQuantum Immunoassay Kit

Product Code. p-7219209
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
Each
Pack of 96
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Product Code. 17195772

Brand: Invitrogen™ A46074

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ELISA

Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

  • High sensitivity-detect low levels of protein with greater sensitivity than traditional methods
  • Broad dynamic range-≥5 logarithmic units, minimizing sample dilutions to ensure they fall within the range
  • Small sample consumption-use 2–5 μL of sample (compared to 75 μL for triplicate wells with other methods)
  • Fast, easy workflow-2 hours from sample to answer, with no wash steps
  • No proprietary instrument to purchase-runs on any real-time PCR instrument

Cardiac Troponin I (Cardiac-specific troponin I, TnI, Troponin I) is an integral inhibitory protein in cardiac muscle that exists as part of a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin-t form, a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, anabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurements of blood TnI and TnT levels is an important diagnostics indicator of heart muscle damage, and can be used to differentiate between angina and myocardial infarction in patients with chest pains. Cardiac Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro. Mutations in the Cardiac Troponin I gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number cardiac troponin I; cardiac troponin I isoform; cardiomyopathy, dilated 2 A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTNI; MGC116817; RCM1; Tn1; TnI; TNNC1; Tnni3; Tpn1; troponin 1, type 3; troponin I type 3 (cardiac); troponin I, cardiac 3; troponin I, cardiac muscle; troponin I, cardiac muscle-like; troponin I3, cardiac type
Assay Range 0.32 to 25,000 pg/mL
Assay Sensitivity <0.3 pg/mL
Conjugate FAM™
Product Type ProQuantum
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) qPCR Instruments
Gene ID (Entrez) 7137
Gene Symbol TNNI3
Kit Contents Assay Dilution Buffer, Antibody-Conjugate A, Antibody-Conjugate B, Antibody-Conjugate Dilution Buffer, Standard, Master Mix, Ligase
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Storage Requirements -20°C
Target Cardiac Troponin I
Test Time 30 mins
Total Assay Time 2 hrs
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