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Description
The Human VEGFR2/KDR/Flk-1 ELISA Kit (Colorimetric) measures human VEGFR2/KDR/Flk-1 in cell culture supernatant, serum, plasma (edta, citrate, heparin).
Specifications
Specifications
| Assay Range | 78 - 5000pg/mL |
| Assay Sensitivity | 7.0pg/mL |
| Product Type | ELISA Kit |
| Sample Type | Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum |
| Specificity | The assay detects both natural and recombinant human VEGFR2/KDR/Flk-1. Cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human VEGFR2/KDR/Flk-1 positive sample. There was no cross reactivity detected, notably not with VEGF-R1 at physiological concentrations and not with VEGF-R3/FLT-4. There was no interference detected, notably not for VEGF-A and VEGF-C. |
| Target Species | Human |
| For Use With (Application) | ELISA |
| Gene ID (Entrez) | 3791 |
| Gene Symbol | KDR |
| Kit Contents | Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to human VEGF-R2/KDR, Biotin-Conjugate anti-human VEGF-R2/KDR monoclonal antibody, Streptavidin-HRP, Human VEGF-R2/KDR Standard lyophilized, 10ng/mL upon reconstitution, Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA), Wash Buffer Concentrate 20x (PBS with 1% Tween 20), Substrate Solution (tetramethyl-benzidine), Stop Solution (1M Phosphoric acid), Adhesive Films |
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