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Syrian Hamster IgG Isotype Control, Biotin, eBioscience™, Invitrogen™

Syrian Hamster Isotype Control

79.50€ - 190.00€

Specifications

Antigen Syrian Hamster IgG
Concentration 0.5 mg/mL
Applications Control, Flow Cytometry, Immunohistochemistry
Conjugate Biotin
Form Liquid
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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15279819
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Affymetrix eBioscience
13-4914-85
500 μg
190.00€
500µg
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Estimated Shipment: 07-05-2024
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15269819
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Affymetrix eBioscience
13-4914-81
50 μg This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.
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Description

Description

Description: This hamster IgG fraction was purified by affinity chromatography from normal Golden Syrian hamster serum. Applications Reported: Golden Syrian hamster IgG isotype control has been reported for use in surface and intracellular flow cytometric analysis, and immunohistochemical staining. Applications Tested: This Golden Syrian hamster IgG isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. Isotype control should be used at the same concentration as experimental antibody. Filtration: 0.2 μm post-manufacturing filtered.

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
Specifications

Specifications

Syrian Hamster IgG
Control, Flow Cytometry, Immunohistochemistry
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
IgG
Affinity chromatography
0.5 mg/mL
Biotin
Syrian Hamster
Not Applicable
IgG; Immunoglobulin G; ImmunoglobulinG
Isotype Control (Primary)
4° C, store in dark, DO NOT FREEZE!
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For Research Use Only.