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  5. Invitrogen™ Equine IL-1RA ELISA Kit

Invitrogen™ Equine IL-1RA ELISA Kit

Product Code. p-7230679
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
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Product Code. 15434883

Brand: Invitrogen™ EEIL1RN

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Description

ELISA

The Equine Interleukin-1 Receptor Antagonist (Eq IL-1RA) ELISA quantitates Eq IL-1RA in equine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Eq IL-1RA. Principle of the method The Equine IL-1RA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number O18999
Assay Range 12.29 to 3000 pg/mL
Assay Sensitivity 10 pg/mL
Calibration Range 10 pg/mL to 3000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cell Structure & Organelle Proteins
Sample Type Plasma, Serum, Supernatant
Specificity The sandwich ELISA antibody pair detects Equine IL-1 RA.
Sufficient For 96 Assays
Target Species Equine
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 100034236
Gene Symbol IL-1 RA
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Extracellular Matrix & Cell Adhesion
Sample Volume Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym IL-1 RA
Target IL1RA
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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