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  5. Invitrogen™ Bovine IL-2 ELISA Kit

Invitrogen™ Bovine IL-2 ELISA Kit

Product Code. 17844040
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Produktkod. 17844040

missing translation for 'mfr': Invitrogen™ EBIL2X10

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Beskrivning

ELISA

The Bovine Interleukin 2 (Bov IL-2) ELISA quantitates Bov IL-2 in bovine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Bov IL-2. Principle of the method The Bovine IL-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.
TRUSTED_SUSTAINABILITY

Specifikationer

Accession Number P05016
Assay Range 0.273 to 200 ng/mL
Assay Sensitivity 0.2 ng/mL
Calibration Range 0.2 ng/mL to 200 ng/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity Detects bovine IL-2 in. this Sandwich ELISA. It shows less than 0.2% cross-reactivity with the recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant cotton rat IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant feline IL-2.
Sufficient For 10 x 96 assays
Target Species Bovine
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 280822
Gene Symbol IL2
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Research Discipline Immunology
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym BOS
Target IL-2
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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