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Invitrogen™ CT-GFP Fusion TOPO™ Expression Kit

Product Code. 10472862
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20 reactions
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10472862 20 reactions Each
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Product Code. 10472862 Brand Invitrogen™ Brand No. K482001

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Description

Includes

CT-GFP Fusion Expression kit contain two boxes: 1. TOPO cloning box with pcDNA 3.1/CT-GFP TOPO cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid.

Designed to allow fusion of protein of interest to Cycle 3 GFP protein

  • Provide topoisomerase-I activated pcDNA3.1/CT-GFP-TOPO vector
  • Taq-amplified DNA fragments are ligated into these vectors in easy 5-minute reaction on bench top
  • Vector is designed for high-level expression of transient or stable GFP fusion proteins in wide range of mammalian cells
  • Expression is easily detected in living cells using fluorescence
  • Addition, recombinant proteins expressed from these vector can be detected on western blots using GFP Antiserum

Cell Analysis, Cell-Based Reporter Assays, Cellular Imaging, Constitutive Expression, Enzyme and Protein Activity Assays, Fluorescent Protein (e.g. GFP) Assays, Fluorescent Protein Assays (GFP), Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Reporter Assays

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type TOPO Expression Kit
Reporter Gene GFP (Cycle 3)
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. 2. One Shot™ TOP10 Chemically Competent E. coli, S.O.C. medium, and supercoiled control plasmid. Store at -80°C. Kits are guaranteed stable for 6 months when properly stored
Protein Tag GFP (Cycle 3)
Cloning Method TOPO-TA
Quantity 20 reactions
Vector TOPO-TA Vectors
Product Line TOPO
For Use With (Application) Reporter Assays
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For Research Use Only. Not for use in diagnostic procedures.

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