Invitrogen™ Exosome-Streptavidin Isolation/Detection Reagent

Exosome-Streptavidin Isolation/Detection Reagent, when combined with your choice of biotinylated primary antibody, enables the purification of exosomes (also called extracellular vesicles and multi-vesicular bodies) that can then be detected using techniques such as flow cytometry, electron microscopy, or Western blotting. The exosomes must be pre-enriched before isolation. This can be done by ultracentrifugation, or by using the fast and efficient Total Exosome Isolation (from cell culture media) reagent.

• Maximum flexibility with your choice of biotinylated antibody
• See your sample during handling
• Easily scalable protocol
• Detect exosomes via flow cytometry, in less than 1 hour
• Utilizes Dynabeads magnetic separation technology
• Allows for easy purification of pre-enriched exosomes from cell culture media and then move on to detect the purified exosomes via techniques such as flow cytometry, electron microscopy, or Western blotting
• Monodispersed and relatively large Dynabeads (4.5 μm in diameter) allow for a clear and defined FFC/SSC typically in less than 1 hour
• Magnetic handling also allows sample to be visualized due to the light brown color of beads

Good Mixing is Critical

• For successful exosome isolatio,n it is important to use a mixer that tilts and rotates to ensure that beads do not settle in tube
• Avoid end-over end rotation for small sample volumes (e.g., 100 μL)
• Please see user manual below for more guidelines