missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. DNA Vectors
  5. 10729364

Invitrogen™ Flp-In™ Complete System

Product Code. 10729364
Change view
Click to view available options
Quantity:
1 kit
Unit Size:
Each
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Product Code. Quantity unitSize
10729364 1 kit Each
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
This item is not returnable. View return policy
Product Code. 10729364 Supplier Invitrogen™ Supplier No. K601001

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Includes

The Flp-In Complete System includes the Core Kit (Cat. No. K601002), 1g Zeocin antibiotic, and 1g hygromycin B. All vectors are supplied supercoiled and lyophilized.

Allows integration and expression of gene of interest in mammalian cells at specific genomic location

  • Introduce of Flp Recombination Target (FRT) site into genome of mammalian cell line of choice
  • Expression vector containing gene of interest is then integrated into genome via Flp recombinase-mediated DNA recombination at FRT site
  • Major components include Flp-In. target site vector, pFRT/lacZeo, for generation of host cell line containing integrated FRT site
  • Expression plasmid containing a FRT site linked to hygromycin resistance gene for Flp recombinase-mediated integration and selection of stable cell line expressing gene of interest under control of human cytomegalovirus (CMV) immediate-early enhancer/promoter
  • Flp recombinase expression plasmid, pOG44, for expression of Flp recombinase under control of human CMV promoter
  • Control expression plasmid containing chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into the Flp-In. host cell line, expresses CAT

Bioproduction, Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Stable Cell Line Development, Targeted Integration

TRUSTED_SUSTAINABILITY

Specifications

Constitutive or Inducible System Constitutive
Delivery Type Transfection
Promoter CMV
Product Type Flp-In Complete System
Selection Agent (Eukaryotic) Zeocin™, Hygromycin
Content And Storage The Flp-In™ Complete System includes the Core Kit (K601002), 1 g Zeocin™ antibiotic, and 1 g hygromycin B. All vectors are supplied supercoiled and lyophilized. Store hygromycin at +4°C. Store all other components at -20°C. Zeocin™ antibiotic should be protected from exposure to light. All reagents are guaranteed stable for 6 months when properly stored.
Protein Tag Untagged
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pFRT
Product Line Flp-In, pcDNA
For Use With (Application) Stable Cell Line Development, Targeted Integration
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.