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Invitrogen™ NativePure™ Lentiviral Gateway™ Vector Kit
Description
Includes
pLenti6/capTEV-NT-DEST1 (N-terminal vector), pLenti6/capTEV-CT-DEST (C-terminal vector), and pLenti6/capTEV-CT-GW/ARP2 (C-terminal) control vector.
- Allow N- or C- terminal fusion of recombinant protein of interest to capTEV± affinity purification tag
- Gateway vectors designed to express protein of interest in multiple expression systems
- Lentiviral vectors with capTEVTag that allow in vivo biotinylation of protein of interest
- Streamlined purification protocols with Streptavidin Agarose
Protein and Peptide Purification,Protein Complex Isolation and Analysis,Protein Sample Preparation and Protein Purification,Protein-Protein Interactions,Proteins, Expression, Isolation and Analysis
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Lentiviral |
| Promoter | CMV |
| Selection Agent (Eukaryotic) | Blasticidin |
| Content And Storage | Store the 293FT Cell Line in liquid nitrogen and the One Shot Stbl3 Chemically Competent E. coli at -80°C. Store Streptavidin Agarose, 10% NP-40, NativePure± 5X Lysis/Binding Buffer, NativePure± 10X TEV Cleavage Buffer, Lipofectamine 2000, NativePure± Columns, and NativePure± Concentrators at +4°C. Store the NativePure Lentiviral Gateway Vector Kit, AcTEV± Protease, 100mM DTT, Gateway LR Clonase Enzyme Mix, ViraPower packaging Mix, and Blasticidin at -20°C. All components are guaranteed stable for 6 months when properly stored. |
| Protein Tag | BioEase Tag, His Tag (6x), V5 Epitope Tag |
| Quantity | 1 Kit |
| Vector | pLenti |
| For Use With (Application) | Protein-Protein Interaction Studies, Viral Expression |
For Research Use Only. Not for use in diagnostic procedures.
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