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Invitrogen™ PureLink™ Genomic DNA Mini Kit

No detectable contamination from carryover of protein or guanidine

Brand:  Invitrogen™ K182001

173.70 EUR valid until 2022-12-31
Use promo code "18380" to get your promotional price.



Additional Details : Weight : 0.56000kg


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Product Code. 10053293

  • 193.00€ / Each

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Description

Description

PureLink Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format.

  • Kit flexibility—one kit works with a variety of sample types and sizes
  • Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
  • Improved design—optimized spin-column design and buffer formulation for better yield and purity
  • Multiple sample sources, one kit—Optimized components and protocols

  • PureLink Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use
  • Columns are newly configured to bind and release DNA more efficiently
  • Each sample source uses specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination
  • Chaotropic salt buffer promotes stable binding of DNA to the column resin and powerful wash buffers remove all traces of protein and salt
  • DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage
Higher yields and purity
  • Expect high yields of high-purity gDNA (determined by A260/A280 measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells
  • Enhanced PureLink lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∽1.9 for both A260/A280 and A260/A230

DNA & RNA Purification & Analysis, DNA Extraction, Epigenetic Sequencing, Genomic DNA Purification, Methylation Analysis by Sequencing, SOLiD™ Next-Generation Sequencing, SOLiD™ Next-Generation Sequencing Sample Purification, Sample & Library Preparation for SOLiD™ Next-Generation Sequencing, Sequencing, gDNA from Animal Tissues & Cells, gDNA from Bacteria, gDNA from Blood (Medium-Scale, 100μL-1mL)

Specifications

Specifications

• 10 ml PureLink™ Genomic Lysis/Binding Buffer
• 9 ml PureLink™ Genomic Digestion Buffer
• 10 ml PureLink™ Genomic Wash Buffer 1
• 7.5 ml PureLink™ Genomic Wash Buffer 2
• 10 ml PureLink™ Genomic Elution Buffer
• 1 ml RNase A (20 mg/ml)
• 1 ml Proteinase K (20 mg/ml)
• 50 each PureLink™ Spin Columns with Collection Tubes
• 100 PureLink™ Collecion Tubes (2.0 ml)

This kit contains enough reagents for 50 DNA preps. Store all components at room temperature. For long-term storage, Proteinase K and RNase A may be stored at 4°C.
Silica Spin Column
Mini
Not High-throughput Compatible (Manual)
100 µl - 1 ml Blood Samples ( Medium-Scale)
Genomic DNA Mini Kit
Room Temperature
Microcentrifuge Spin Column
Tissue
Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
50 Preps
PureLink™
50 Preps
Genomic DNA
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • PURELINK GENOMIC DNA MINI 50 PREPS

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : guanidine hydrochloride, proteinase

SDS
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For Research Use Only. Not for use in diagnostic procedures.