Invitrogen™ PureLink™ Genomic Wash Buffer I

PureLink Genomic DNA Mini Kit and PureLink 96 Genomic DNA Kit enable high-yield, high-purity DNA extractions from wide variety of sample types, including blood, tissues, cells, bacteria, swabs, and blood spots, in a familiar silica spin-column or semi-skirted 96-well plate format.

• Obtain higher gDNA yields and purity—optimized spin-column membrane and buffer formulations
• Save time and money—use with large range of sample sizes (e.g., 100 μL to 1 mL blood); 96-well version does not require special equipment
• Increase flexibility—one kit works with variety of sample types

How it works

• PureLink Genomic DNA Mini Kit protocol improves upon easy, familiar spin-column methods currently in use
• Columns are newly configured to bind and release DNA more efficiently
• Samples are lysed in optimized buffer formulated to enhance proteinase K activity and eliminate protein contamination
• Powerful wash buffers remove all traces of protein and salt
• DNA is eluted in low-salt buffer to allow for pH stabilization of the DNA in storage

For higher throughput, use the PureLink 96 Genomic DNA Kit

• In this 96-well version, sample processing is performed with either vacuum manifold or centrifugation at only 2,250 g, allowing more flexibility in centrifuge choice
• The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations

Higher yields and purity

• With the PureLink Genomic DNA Kits, get greater yields of higher-purity gDNA (determined by A260/A280 measurements) with all sample types - from bacteria to tissues to blood to cells
• PureLink 96 Genomic DNA Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood
• A260/A280 and A260/A230 ratios of PureLink gDNA are higher than those obtained with other kits, indicating no contamination carryover of protein or guanidine using the enhanced PureLink Lysis and Wash Buffers