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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit
The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli.
Brand: Invitrogen™ K210004
219.85 EUR valid until 2024-04-30
Use promo code "21741" to get your promotional price.
Additional Details : CAS : 9048-46-8 Weight : 2.00000kg
Description
The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 μg plasmid DNA from 15–25 mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.
- Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
- High-purity, low-endotoxin product- pure enough for mammalian cell transfection
- Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
Anion-exchange chromatography for plasmid purification
- PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
- The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
- Plasmid preparations can typically be completed in less than 2 hours
Contaminant-free DNA
- Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
- Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A260/A280 ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
- Endotoxin levels are typically within 0.1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection
- Purification time is two hours
Specifications
| • 100 mL Resuspension Buffer (R3) • 550 μL RNase A • 100 mL Lysis Buffer (L7) • 100 mL Precipitation Buffer (N3) • 250 mL Equilibration Buffer (EQ1) • 500 mL Wash Buffer (W8) • 125 mL Elution Buffer (E4) • 15 mL TE Buffer • 25 HiPure Columns • 5 Column Holders Store all components at room temperature. |
|
| Anion Exchange Resin | |
| 5 mL | |
| Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription | |
| 25 Preps | |
| 100-200 μg (Medium-Scale) Plasmid DNA | |
| Plasmid MidiPrep Kit | |
| Transfection Grade | |
| BAC DNA, Plasmid DNA | |
| 2 hr. |
| Column | |
| Bacterial Culture | |
| BAC DNA, Plasmid DNA | |
| Not High-throughput Compatible (Manual) | |
| <40kb, Low Copy Plasmid, High Copy Plasmid, BAC | |
| PureLink™ | |
| 25 Preps | |
| Room Temperature | |
| PureLink™ | |
| 350 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- PureLink HiPure Plasmid Midiprep Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
Hazard Statement
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
Supplemental information
- MIXTURE LIST-Contains : acetic acid, Sodiumlauryl sulfate, Sodium hydroxide
For Research Use Only. Not for use in diagnostic procedures.