Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit

Description

The PureLink HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350µg plasmid DNA from 15–25mL bacterial culture, at purity that is comparable to that achieved by two passes through cesium chloride gradients.

• Labor and time saving- no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product- pure enough for mammalian cell transfection
• Versatility- purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs

• PureLink HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to level equivalent to two passes through CsCl gradients
• The resin combines excellent capacity with fast flow rate, high resolution, high yield, and efficient endotoxin removal
• Plasmid preparations can typically be completed in less than 2 hours

• Unlike cesium chloride gradient protocols, PureLink HiPure Plasmid Purification Systems do not use troublesome solvents to work with and to dispose of
• Typically, plasmid DNA prepared using the PureLink HiPure Plasmid Midiprep Kit will have an A₂₆₀/A₂₈₀ ratio of greater than 1.80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications
• Endotoxin levels are typically within 0.1–1 EU/µg, making this plasmid DNA ideal for mammalian cell transfection
• Purification time is two hours

Amplified Fragment Length Polymorphism-AFLP™, Bacterial Artificial Chromosome (BAC) Fingerprinting, DNA and RNA Purification and Analysis, DNA Extraction, DNA Sequencing, Fragment Analysis, Midiprep, Plasmid DNA Purification, Sequencing