Lactoferrin Monoclonal Antibody (B97), eBioscience™, Invitrogen™

Description

Description: This B97 monoclonal antibody reacts with human lactoferrin. This 80-kDa glycoprotein is detectable in the kidney, lung, pancreas, gallbladder, prostate, seminal vesicle, gut, and liver. Lactoferrin is also present in mucosal secretions such as breast milk, tears, and saliva. In immune cells, high levels of lactoferrin can be found within the secondary granules of neutrophils, but not in lymphocytes or monocytes. A member of the transferrin family of proteins, lactoferrin binds two iron molecules to mediate its transport and storage. Lactoferrin plays an important role in host defense against pathogens and can regulate adaptive immune responses. Receptors for lactoferrin are present on the surface of lymphocytes, granulocytes, monocytes, mast cells, epithelial, and endothelial cells. Applications Reported: This B97 antibody has been reported for use in immunoblotting (WB) and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This B97 antibody has been tested using high pH antigen retrieval immunohistochemistry on formalin-fixed paraffin embedded human infiltrating ductal carcinoma and by western blot on reduced human saliva lysate. For immunohistochemistry, this antibody can be used at less than or equal to 10 μg/mL. For western blotting, the B97 antibody can be used at less than or equal to 1 μg/mL.

Lactoferrin (LF) is an iron binding glycoprotein. LF consists of a single polypeptide chain (approximately 80 kDa) folded into two structurally homologous lobes, each of which can reversibly bind one ferric ion (Fe3+). LF is found in external fluids, including milk and mucosal secretions, and is a prominent component of secondary granuals of neutrophils. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. LTF acts as a major first line defense against microbial infections, partly due to its ability to efficiently bind to and hence remove Fe3+ from the environment, but studies have also shown that LTF possesses bactericidal properties and proteolytic activity, capable of cleaving arginine-rich sequences within microbial proteins. Three isoforms of LTF have been identified: LTF-alpha, LTF-beta and LTF-gamma and receptors for LTF are expressed in the gastrointestinal tract, by macrophages, lymphocytes, neutrophils and platelets and also by some bacteria.