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Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System
Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the
Brand: Invitrogen™ T1033
Additional Details : Weight : 0.00450kg
Includes: Two reaction buffers
Description
- Homogeneous assay format allows detection of firefly luciferase in presence of normal culture media without removal of media and without an additional cell lysis step, providing easiest and most streamlined assay procedure possible
- Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
- Wide dynamic range of firefly luciferase assay lets the user measure enzyme level accurately from femtogram to nanogram range
- Assay sensitivity is 100- to 1,000-fold better than with either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
- Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
- Non-radioactive reporter gene assay kit eliminates radioisotopes
- Assay can be completed in about one hour, providing fast assay turnaround
- Assay Type: Luciferase Assay
- Compatible Cells: Mammalian Cells
- Detection Method: Chemiluminescent
- For Use With Equipment: Luminometer (Microplate)
- Form: Liquid
- Format: 96-well plate
- Readout: End Point
- Sample Type (Specific): Cell Cultures
- Substrate: Luciferin
- Substrate Properties: Chemical Substrate
- Substrate Type: Luciferase Substrate
Specifications
Luciferase Reporter Gene Assay System | |
Luminometer (Microplate) | |
This kit contains two reaction buffers. Store the product at -20°C. |
|
High-throughput Compatible | |
Cell Cultures | |
Luciferase Substrate | |
Luciferase | |
Enzyme Assay | |
Liquid |
Chemiluminescent | |
Luciferase Assay | |
Mammalian Cells | |
End Point | |
Luciferin | |
Chemical Substrate | |
Luc-Screen™, NovaBright™ | |
1000 Assays |
For Research Use Only. Not for use in diagnostic procedures.