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Ly-6A/E (Sca-1), PE-Cyanine5, clone: D7, eBioscience™

Rat Monoclonal Antibody

Marque:  Affymetrix eBioscience 15-5981-81

Informations supplémentaires : Poids : 0.25000kg

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Code produit. 15360540

  • 163.00€ / 50µg


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Description

Description

Description: The D7 monoclonal antibody reacts with mouse Sca-1, an 18kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.Applications Reported: This D7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This D7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Spécification

Spécification

Ly-6A/E (Sca-1)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P05533, Q64253
lymphocyte antigen 6 complex, locus A/E
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
D7
PE-Cyanine5
Ly6e
Liquid
Ly6a, Ly6e
IgG2a, kappa, kappa
50μg
4° C, store in dark, DO NOT FREEZE!
Primary
110454, 17069
Vidéos

For Research Use Only.