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  6. Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)

Product Code. 30245027
Click to view available options
Quantity:
100 Reactions
120 μg
1600 μg
2000 μL
Product Type:
Lyo-ready RPA Kit
Lyo-ready T4 Gene 32 Protein
Lyo-ready T4 UvsX Protein
Lyo-ready T4 UvsY Protein
Reaction Buffer
Unit Size:
100 reactions
120µg
1600µg
2000µL
This item is not returnable. View return policy

Product Code. 30245027

Brand: Invitrogen™ A72126

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This item is not returnable. View return policy

Beskrivning

Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.

Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.

Features of Lyo-ready RPA products

  • Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
  • Robust and specific RNA/DNA amplification
  • Amplification from inhibitor-containing samples
  • Fast target amplification in as little as 20 minutes
  • Ability to easily optimize RPA or RT-RPA reactions

Kit components

The Lyo-ready RPA Kit includes all required components for an RPA reaction:

  • Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
  • Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
  • Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
  • Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
  • Optimized reaction buffer, magnesium chloride, and dNTPs
TRUSTED_SUSTAINABILITY

Specifikationer

Product Type Reaction Buffer
Form Liquid
For Use With (Application) RPA, RT-RPA
Quantity 2000 μL
Research Category Nucleic Acid Amplification
Content And Storage 2X RPA Reaction Buffer, 2 mL, Store at -25°C to -15°C.
Optimal Reaction Temperature 42°C
Shipping Condition Dry Ice
Concentration 2X
Reaction Time Up to 20 min.

For Research Use Only. Not for use in diagnostic procedures.

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