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  5. Invitrogen™ Mouse MCP-1/CCL2 Uncoated ELISA Kit

Invitrogen™ Mouse MCP-1/CCL2 Uncoated ELISA Kit

Product Code. p-7203461
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Quantity:
10 x 96 Tests
20 x 96 Tests
Unit Size:
Each
Pack of 10
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Product Code. 15591137

Brand: Invitrogen™ 88739188

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Description

ELISA

The Mouse Monocyte Chemoattractant Protein-1/CCL2 (MCP-1) Uncoated ELISA Kit contains pre-matched antibody pairs, and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse MCP-1. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P10148
Assay Range 15 to 2,000 pg/mL
Assay Sensitivity 15 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 20296
Gene Symbol AI553453,CCP-1/C11,CCP1,Ctla-1,Ctla1,GZB
Kit Contents Capture Antibody: Pre-titrated, Purified antibody, Detection Antibody: Pre-titrated, Biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye HRP
Quantity 10 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym Monocyte chemotactic protein 1
Target CCL2 (MCP-1)
Test Time 1 hrs 45 mins
Total Assay Time 24 hrs 30 mins
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