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IBA Lifesciences™ MHC I-Strep HLA-A*0201; WT1 (VLDFAPPGA); Fused to Twin-Strep-tag™
MHC Class I molecule fused to a streptavidin derivative and refolded with a peptide.
Brand: IBA Lifesciences™ 6-7099-001
Additional Details : Weight : 0.00200kg
Description
MHC I-Strep HLA-A*0201; WT-1 (37-45) (VLDFAPPGA) is a recombinantly expressed MHC Class I molecule that presents a peptide from Wilms tumor antigen 1 (WT1). Our MHC I Streptamer™ approach is based on our Strep-tag™ technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag™ fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin™ backbone, resulting in the formation of MHC I Streptamers.
For multimerizing MHC I-Streps, Strep-Tactin™ TACS Agarose columns, Strep-Tactin™ Magnetic Microbeads or fluorophore-conjugated Strep-Tactin™ can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements.
- Addition of biotin reverses multimerization of MHC Class I molecules, leading to dissociation from target cells
- Resulting label-free cells are highly viable and fully functional
Specifications
| Dissolved in DPBS | |
| 10 μg | |
| Human |
| Cell Isolation | |
| Antigen-specific T Cell | |
| 0.2 μg per 2 x 106 Total Cells |