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  5. Invitrogen™ Human MMP1-Cell Lysate ELISA Kit

Invitrogen™ Human MMP1-Cell Lysate ELISA Kit

Product Code. 15455073
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Quantity:
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Product Code. Quantity unitSize
15455073 96 Tests 1 set
17175509 5 x 96 Tests Each
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Product Code. 15455073

Brand: Invitrogen™ EHMMP1CL

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Description

ELISA

The Human Matrix MetalloProteinase 1 (Hu MMP1) ELISA quantitates Hu MMP1 in human cell and tissue lysate. The assay will exclusively recognize both natural and recombinant Hu MMP1. Principle of the method The Human MMP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
TRUSTED_SUSTAINABILITY

Especificaciones

Accession Number P03956
Assay Range 24.69 to 18,000 pg/mL
Assay Sensitivity 8 pg/mL
Calibration Range 8 pg/mL to 18000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Cell Structure & Organelle Proteins
Sample Type Cell Lysates
Specificity The antibody pair provided in this kit recognizes human MMP-1.
Sufficient For 96 Assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4312
Gene Symbol CLG,CLGN
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Cell Lysis Buffer, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Extracellular Matrix & Cell Adhesion
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym CLG, CLGN
Target MMP1
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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