Invitrogen™ Amplex™ Red Neuraminidase (Sialidase) Assay Kit

The Amplex Red Neuraminidase (Sialidase) Assay Kit provides a sensitive and simple method for detecting neuraminidase activity in serum and in purified enzyme systems using either a fluorescence microplate reader or fluorometer. Neuraminidase (also known as sialidase) is a very common enzyme that hydrolyzes terminal sialic acid residues on polysaccharide chains, most often exposing a galactose residue. Neuraminidase plays a key role in the invasion of target cells and the subsequent replication of the influenza virus, making neuraminidase an important target for influenza drug development. The Amplex Red Neuraminidase (Sialidase) Assay Kit provides an ultrasensitive method for detecting neuramimidase activity. This assay utilizes Amplex Red reagent to detect hydrogen peroxide generated by galactose oxidase oxidation of desialiated galactose, the end result of neuraminidase action. Then the hydrogen peroxide, in the presence of horseradish peroxidase (HRP), reacts in a 1:1 stoichiometric ratio with Amplex Red reagent to generate the red-fluorescent oxidation product resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples.

• Detect neuraminidase levels as low as 0.2mU/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference
• Detect neuraminidase activity in biological samples such as serum
• Custom assay design and packaging are also available

Use Amplex Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent. The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours.

Enzyme and Protein Activity Assays, Glycosidase, Neuraminidase, and Related Enzyme Activity, Proteins, Expression, Isolation and Analysis