Invitrogen™ Staphylococcus aureus (Wood strain without protein A) BioParticles™, Alexa Fluor™ 594 conjugate

Fluorescent products have been employed to study phagocytosis by fluorescence microscopy, quantitative spectrofluorometry, and flow cytometry

Unlike the fluorescence of fluorescein-labeled BioParticles conjugates, which is partially quenched in acidic environments, the fluorescence of the Alexa Fluor BODIPY™ FL, tetramethylrhodamine and Texas Red™ dye conjugates is uniformly intense over the pH range from 3 to 10.

• Label (Ex/Em): Alexa Fluor 594 (approx. 590/617nm)
• Particle: S. aureus (Wood strain, without protein A)
• Provided as lyophilized powders
• approximately 3 x 10⁸ S. aureus particles per mg solid and approximately 2 x 10⁷ zymosan particles per mg solid
• Conjugates can be reconstituted in buffer of choice for use in phagocytosis assays
• Conjugates that are bound to the surface of the cell (but not internalized) can be quenched by ethidium bromide, trypan blue, or other quenchers
• Fluorescent BioParticles conjugates may be effective as flow cytometry calibration references when sorting bacteria and yeast mutants
• Small particles may also be useful references for light scattering studies because their sizes and shapes differ in characteristic ways

Cell Analysis, Cell Structure, Cell Viability, Proliferation and Function, Cellular Imaging, Endocytosis, Exocytosis and Phagocytosis, Immunofluorescence (IF), Immunofluorescence Staining and Detection, Receptors