Invitrogen™ E-selectin (Soluble) Monkey ELISA Kit

Beinhaltet

• Aluminum pouch(es) with a Microwell Plate coated with monoclonal antibody to monkey sE-selectin
• HRP-Conjugate anti-monkey sE-selectin monoclonal antibody
• Monkey sE-selectin Standard lyophilized, 120 U/ml upon reconstitution
• Sample Diluent
• Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
• Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
• Substrate Solution (tetramethyl-benzidine)
• Stop Solution (1M Phosphoric acid)
• Blue-Dye
• Green-Dye
• Adhesive Films

For the quantitative detection of monkey sE-selectin.

The Monkey Endothelial Leukocyte Adhesion Molecule (Mk e-Selectin/ELAM-1) ELISA quantitates Mk ELAM-1 in baboon serum or cell culture medium. The assay will exclusively recognize both natural and recombinant Mk ELAM-1. Principle of the method The Monkey ELAM-1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.