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Invitrogen™ Granzyme B Mouse Uncoated ELISA Kit with Plates

Specifically engineered for accurate and precise measurement of Mouse Granzyme B protein levels in serum, plasma, and tissue culture supernant samples.

242.00€ - 544.00€

Specifications

Accession Number P04187
Assay Range 40-5,000 pg/mL
Assay Sensitivity 40 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15122175
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Invitrogen™
88-8022-22
2 x 96 Tests
242.00€
192 tests
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Estimated Shipment: 04-06-2024
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15364821
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Invitrogen™
88-8022-86
10 x 96 Tests
544.00€
Each
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Estimated Shipment: 04-06-2024
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Description

Description

The Mouse Granzyme B Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse Granzyme B. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Granzyme B Mouse Uncoated ELISA Kit (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA).
Specifications

Specifications

P04187
40 pg/mL
ELISA Kit
Mouse
Colorimetric Microplate Reader
AI323594,HC11,JE,MCAF,MCP-1,MCP1,SMC-CF,Scya2,Sigje
HRP
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL
GrzB, GzB
1 hr. 45 min.
40-5,000 pg/mL
Biotin
Plasma, Serum, Supernatant
ELISA
14939
Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86)
RUO
2°C to 8°C
Mouse
24 hr. 30 min.
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