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Invitrogen™ IL-1 beta Mouse Uncoated ELISA Kit with Plates
Specifically engineered for accurate and precise measurement of mouse IL-1 beta protein levels from samples including serum, protein lysate and cell culture supernatants. This ELISA set will recognize the cleaved, mature form and uncleaved pro-form of Mouse IL-1 beta.
221.00€ - 758.00€
Specifications
| Accession Number | P10749 |
|---|---|
| Assay Range | 8-1,000 pg/mL |
| Assay Sensitivity | 8 pg/mL |
| Conjugate | Biotin |
| Product Type | ELISA Kit |
Includes: Capture Antibody: Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant protein for generating standard curve and calibrating samples
10X Coating Buffer . Buffer for plating the Capture Antibody
5X ELISA/ELISPOT Diluent . Buffer for blocking and diluting the Detection Antibody and Enzyme
Enzyme. Pre-titrated Avidin-HRP
Substrate. 1X TMB Solution
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
13598920
|
Invitrogen™
88-7013-22 |
2 x 96 Tests |
221.00€
Pack of 2 |
In Stock
Log in to see stock. |
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|
15580987
|
Invitrogen™
88-7013-76 |
20 x 96 Tests |
This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance. |
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|
15500997
|
Invitrogen™
88-7013-86 |
10 x 96 Tests |
This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance. |
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Description
The Mouse Interleukin-1beta (IL-1b) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-1b. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Mouse IL-1 beta ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA)- To ensure optimal results from the Mouse IL-1 beta ELISA Ready-SET-Go! Set, please only use the components included in the set. Exchanging of components may lead to a change in signal
Specifications
| P10749 | |
| 8 pg/mL | |
| ELISA Kit | |
| Mouse | |
| Colorimetric Microplate Reader | |
| IL-12p35,Il-12a,Ll12a,p35,Il-12b,Il-12p40,Il12p40,p40 | |
| HRP | |
| Cell Lysate,100 μL; Serum, 100 μL; Supernatant, 100 μL | |
| Interleukin-1 beta | |
| 1 hr. 45 min. |
| 8-1,000 pg/mL | |
| Biotin | |
| Cell Lysates, Serum, Supernatant | |
| ELISA | |
| 16176 | |
| Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Enzyme: Pre-titrated Avidin-HRP, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -76; -86) | |
| RUO | |
| 2°C to 8°C | |
| Mouse | |
| 24 hr. 30 min. |
For Research Use Only