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  5. Invitrogen™ Mouse IL-15/IL-15R Complex Uncoated ELISA Kit with Plates

Invitrogen™ Mouse IL-15/IL-15R Complex Uncoated ELISA Kit with Plates

Product Code. 15541077
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Quantity:
10 x 96 Tests
2 x 96 Tests
Unit Size:
Pack of 10
Pack of 2
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Product Code. 15541077

Brand: Invitrogen™ 88721522

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Description

ELISA

The Mouse Interleukin 15/IL-15 Receptor Complex (IL-15/IL-15R) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of mouse IL-15/IL-15R. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-15 (IL-15) is a 14 kDa proinflammatory protein that has been shown to play a role in the activation of neutrophils, dendritic cells, and macrophages, and is essential to the development and survival of NK cells and CD8 T-cells. IL-15 activates Jak/Stat pathways by signaling through a heterotrimeric receptor. Two of the subunits of this receptor, beta and gamma, are shared with the IL-2 receptor, while the alpha subunit is unique to IL-15.Despite the expression of IL-15 mRNA in many cell types, the secreted protein is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and is only secreted in complex with its unique receptor, IL-15R alpha. This chaperoning is required from the ER to Golgi through to secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta/gamma subunits of the receptor, or may be secreted in a soluble form that lacks the transmembrane domain of the receptor. The soluble IL-15/IL-15R complex is produced by mouse dendritic cells, fibroblasts, and macrophages, and demonstrates a 10-100-fold increase in agonistic activity over IL-15 alone.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P48346, Q60819
Assay Range 4-500 pg/mL
Assay Sensitivity 4 pg/mL
Content And Storage Capture Antibody: Pre-titrated, purified antibody
Detection Antibody: Pre-titrated, biotin-conjugated antibody
Standard: Recombinant cytokine for generating standard curve and calibrating samples
Coating Buffer: 10X PBS ELISA Coating Buffer
Assay Diluent: 5X Concentrated
Detection Enzyme: Pre-titrated Avidin-HRP
Substrate Solution: Tetramethylbenzidine (TMB) Solution
Certificate of Analysis: Lot-specific instructions for the dilutions of antibodies and standards
96-Well Plates: Corning Costar Flat-bottom (included with Cat. Nos. ending in -22 and -86)
Conjugate HRP
Immunoassay Kit Format Uncoated ELISA Kit with Plates
Product Type ELISA
Sample Type Serum, Supernatant
Target Species Mouse
For Use With (Application) ELISA
For Use With (Equipment) Absorbance Microplate Reader
Gene Alias AA690181; AI503618; CD215; Il15; IL-15; IL-15 receptor subunit alpha; Il15ra; IL-15RA; IL-15R-alpha; ILN; Interleukin; interleukin 15; interleukin 15 receptor, alpha chain; interleukin-15; Interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; Soluble interleukin-15 receptor subunit alphaAA690181; AI503618; CD215; Il15; IL-15; IL-15 receptor subunit alpha; Il15ra; IL-15RA; IL-15R-alpha; ILN; Interleukin; interleukin 15; interleukin 15 receptor, alpha chain; interleukin-15; Interleukin-15 receptor subunit alpha; sIL-15 receptor subunit alpha; Soluble interleukin-15 receptor subunit alpha;
Gene ID (Entrez) 16168, 16169
Gene Symbol Il15, Il15ra
Kit Contents Capture Antibody: Pre-titrated, purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86), Purified antibody, Detection Antibody: Pre-titrated, HRP-conjugated antibody, Standard: Recombinant protein for generating standard curve and calibrating samples, 10X Coating Buffer: Buffer for plating the Capture Antibody, 5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme, Substrate: 1X TMB Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86)
Label or Dye Biotin
Quantity 2 x 96 Tests
Regulatory Status RUO
Sample Volume Serum 100 μL, Supernatant 100 μL
Storage Requirements 2-8°C
Synonym IL-15 Receptor complex with IL-15
Target IL-15/IL-15R
Test Time 1 hr 45 min
Total Assay Time 24 hr 30 min
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