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Invitrogen™ Myeloperoxidase Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMMPO

432.02 EUR valid until 2024-03-29
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Additional Details : Weight : 0.00400kg

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Product Code. 15485313

  • 648.00€ / 1 set
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Description

Description

The Mouse Myeloperoxidase ELISA quantitates Ms Myeloperoxidase in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Myeloperoxidase. Principle of the method The Mouse Myeloperoxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains with a molecular weight range of 59 - 64 kDa, and two unglycosylated beta chains (MW ∽14 kDa) with total MW ∽150 kDa and a theoretical pI of 9.2. Traditionally, myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities such as periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently, it was shown that myeloperoxidase participates in the initiation and progression of cardiovascular disease. Myeloperoxidase possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. Myeloperoxidase is part of the host defense system of polymorphonuclear leukocytes and myeloperoxidase is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN (polymorphonuclear leukocytes), MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
Specifications

Specifications

P11247
600 pg/mL
Myeloperoxidase ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Myeloperoxidase (MPO) in Samples Using a Microplate Reader
Solid-phase sandwich ELISA
Colorimetric
Other Proteins
This ELISA antibody pair detect mouse MPO. Other species not determined yet
Mouse
Colorimetric Microplate Reader
mKIAA4033
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse Myeloperoxidase precoated 96-well strip plate, lyophilized recombinant mouse myeloperoxidase standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay di
96 Tests
Other Research
2°C to 8°C
Mouse
4 hr. 45 min.
0.61 to 150 ng/mL
0.6ng/mL to 150ng/mL
Myeloperoxidase (MPO), myeloperoxidase (MPO), mouse MPO
Biotin
ELISA Kit
Plasma, Serum, Supernatant
96 Determinations
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
17523
Mouse
<10%
HRP
RUO
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
RP23-449P23.5, mKIAA4033
1 hr. 20 min.
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