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  5. Invitrogen™ Human MSP (MST1) ELISA Kit

Invitrogen™ Human MSP (MST1) ELISA Kit

Product Code. p-7230908
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 15485073

Brand: Invitrogen™ EHMST1

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Description

ELISA

The Human MSP (MST1) ELISA quantitates Hu MSP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MSP. Principle of the method The Human MSP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

MSP (or MST1) contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P26927
Assay Range 8.23 to 2000 pg/mL
Assay Sensitivity 8 pg/mL
Calibration Range 8 pg/mL to 2000 pg/mL
Assay Solid-phase sandwich ELISA
Conjugate HRP
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sufficient For 96 assays
Target Species Human
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4485
Gene Symbol MST1
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Reproduction
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym D3F15S2, DNF15S2, HGFL, MSP, NF15S2
Target MSP
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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