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Invitrogen™ Recombinant Protein G Agarose

Product Code. 11598896
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11598896 5mL
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Product Code. 11598896 Supplier Invitrogen™ Supplier No. 15920010

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Description

Includes

rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol.

For the purification of IgG from most mammalian species and does not bind IgM, IgA, IgE or serum albumin

  • Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol)
  • The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads
  • rProtein G Agarose has a binding capacity of approximately 20mg of human IgG permL of beads
  • rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A
  • The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography
  • To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH
  • Non-IgG components do not bind to the beads and elute in the flow through fraction; Purified IgG is then eluted with a low pH buffer

PCR, PCR and Real-Time PCR

Order Info

Shipping Condition: Wet Ice

TRUSTED_SUSTAINABILITY

Specifications

Column Type Affinity Column
Content And Storage rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol. Store at +4°C.
Separation of Protein
Form Suspension
Shipping Condition Wet Ice
Stationary Phase Protein G
Product Type Agarose Gel

For Research Use Only. Not for use in diagnostic procedures.

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