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Invitrogen™ Resistin Human ELISA Kit

Product Code. 10180403
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 10180403

Brand: Invitrogen™ KHP0051

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Description

Includes

Pre-coated plate, standard, control, detector antibody, anti-rabbit HRP, diluents, wash buffer, chromogen, and stop solution.

Sandwich ELISA Kit

The Human Resistin ELISA quantitates Hu Resistin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Resistin. Principle of the method The Human Resistin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Adipose tissue is an energy reserve in animals and is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice. Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.

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Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q9HD89
Assay Range 0.125-8 ng/mL
Assay Sensitivity 100 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Plasma, Serum, Supernatant
Target Species Human
For Use With (Application) Colorimetric Detection
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 56729
Gene Symbol ADSF,FIZZ31,RSTN,XCP1,RETN
Incubation Time 3.3 hr.
Interassay CV 5.6%
Intraassay CV 3.7%
Kit Contents Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Marker Gene Resistin
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma, 25 μL; Serum, 25 μL; Supernatant, 100 μL
Storage Requirements 2°C to 8°C
Target Human
Test Time 1 hr. 20 min.
Total Assay Time 3 hr. 10 min.
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For Research Use Only. Not for use in diagnostic procedures.

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