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  5. Macherey-Nagel™ NucleoBond™ RNA Soil Mini kit for RNA from soil

Macherey-Nagel™ NucleoBond™ RNA Soil Mini kit for RNA from soil

Product Code. 15858096
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No. of Reactions:
2
10
50
Product Type:
RNA Kit
RNA Kit + DNA Set
Unit Size:
10 reactions
2 reactions
50 reactions
This item is not returnable. View return policy

Product Code. 15858096

Brand: Macherey-Nagel™ 740142.10

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This item is not returnable. View return policy

Description

Easy handling and superior speed in RNA purification from soil samples for qRT-PCR analysis, as well as parallel preparation of DNA. Complete kit uses anion exchange chromatography and gravity flow columns combined with NucleoSpin Bead Tubes Type A (included).

NucleoBond™ RNA Soil Mini kit enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.

  • Anion exchange technology to optimize RNA yield and purity—suitable for qRT-PCR analysis
  • Combination of mechanical homogenization and chemical lysis allows processing of large samples
  • Parallel preparation of RNA and DNA in one hour*
  • Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
  • Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples

* DNA isolation requires the DNA Set for NucleoBond™ RNA Soil Mini, sold separately (man. part no. 740143.10 or 740143.50) or in a combo kit

Specifications

Format Gravity Flow Column
Isolation Technology Anion Exchange Chromatography, Gravity Flow Columns, NucleoSpin Bead Tubes
Elution Volume Elution volume: 50-100 μL
Sample Type Soil
Efficiency Binding capacity: 30 μg
For Use With (Equipment) Centrifuge for microcentrifuge tubes
For Use With (Application) RNA/DNA isolation from soil for qRT-PCR
Includes NucleoBond Soil Mini Columns, NucleoSpin Bead Tubes Type A, NucleoSpin RNA columns, Buffers
No. of Reactions 10
Product Type RNA Kit
Purity A260/A280: 1.5 - 2.0
Sample Size 0.25 to 0.5 g
Test Sensitivity RNA integrity number (RIN): > 7.0
Test Time 60 min/12 preps (RNA)
Yield 0.25 to 2.5 μg
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Safety and Handling

compliance-icons compliance-icons
Product Identifier
  • NucleoBond RNA Soil Mini (10)
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H319-Causes serious eye irritation.
 Supplemental information
  • MIXTURE LIST-Contains : EDTA, tris(hydroxymethyl)aminomethane, sodium chloride, dodecyl sulphate sodiumsalt, potassium chlo
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