Macherey-Nagel™ NucleoSpin™ Blood L, Midi kit for DNA from blood

NucleoSpin™ Blood L is for the rapid, medium-scale preparation of highly pure genomic DNA from up to 2mL whole blood, serum, plasma or other body fluids. Buffy coat up to 1mL can be processed easily. If enrichment of leukocytes is not possible, e.g., after freezing of blood which leads to lysis of cells, large volumes of blood samples have to be processed. The obtained DNA can be used directly as template for PCR* reactions or can be subjected to endonuclease digestion. Due to the high binding capacity of the NucleoSpin™ Blood L columns, which is about 250μg of genomic DNA, the kit allows preparation of pure genomic DNA from up to 2mL of human or animal whole blood with an A_260/280 ratio between 1.70 and 1.90.Typical DNA yields – depending on the concentration of leukocytes in the respective blood sample – are in the range of 20μg to 30μg per mL of healthy human blood. Blood treated either with EDTA, citrate, heparin or CPDA can be used. The procedure is optimised for a sample volume of 0.5mL to 2mL. Time for preparation of genomic DNA from blood with NucleoSpin™ Blood L is less than 1 hour. Kit components: NucleoSpin™ Blood L columns, 15mL collecting tubes, 15mL elution tubes, buffers, proteinase K.

• Silica membrane technology
• Yield: 40 to 60μg
• Sample material: ≤200μL/5 x 10⁶ cells
• Elution volume: 100μL
• Binding capacity: 60μg
• Preparation time: 30 min./prep
• Format: mini spin column
• No use of organic solvents For rapid isolation of genomic DNA from blood, serum, plasma or other body fluids.