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OPTIZYME™ BamHI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8005-5

Additional Details : Weight : 0.01000kg

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Product Code. 11974842

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...G^G A T C C...3'
3'...C C T A G^G...5'

Conditions for 100% Activity:

  • 1X OPTIZYME Buffer BamHI: 10mM Tris-HCl (pH 8.0 at 37°C), 5mM MgCl2, 100mM KCl, 0.02% Triton X-100 and 0.1mg/ml BSA.
  • Incubate at 37°C
Supplied with: 10X OPTIZYME BamHI Buffer
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%™
  • Buffer 2: 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%™
  • Buffer 5: 50 - 100%™
™star activity occurs at greater than 5-fold overdigestion (5 units × 1 hour)
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage:
More than 95% of DNA fragments can be ligated and recut after a 50-fold overdigestion with BamHI.
Digestion of Agarose-embedded DNA:
A minimum of 5 units of BamHI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
Note: Low salt, high glycerol (>5%) concentrations, pH >8.0 or a large excess of enzyme may result in star activity.
Compatible Ends: BclI, BglII, Bsp143I, MboI, PsuI.
Methylation Effects:
Dam: completely overlaps - no effect.
Dcm: may overlap - no effect.
CpG: may overlap - no effect.
EcoKI: never overlaps - no effect.
EcoBI: never overlaps - no effect.

Specifications

Specifications

10 U/μL
37°C
>95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BamHI
Keep container tightly closed
G.GATCC
Liquid
25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
7.4
10mM Tris HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
12,500 U
BamHI
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