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OPTIZYME™ BclI, Fisher BioReagents™
Brand: Fisher Bioreagents BP8053-1
Additional Details : Weight : 0.01000kg
Description
5'...T^G A T C A...3'
3'...A C T A G^T...5'
Supplied with: 10X OPTIZYME Buffer 2
Conditions for 100% Activity:
- 1X OPTIZYME Buffer 2: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/ml BSA
- Incubate at 55°C
- Buffer 1: 20 - 50%
- Buffer 2: 100%
- Buffer 3: 20 - 50%
- Buffer 4: 100%™
- Buffer 5: 20 - 50%
Storage Buffer:
- 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
- Incubation at 37°C results in 50% activity.
- Greater than 15-fold overdigestion with BclI may result in star activity.
- Assayed using lambda DNA (dam-)
- BclI is blocked by dam methylation. To avoid dam methylation, use a dam-, dcm- strain.
Methylation Effects:
Dam: Completely overlaps - blocked
- TGm6ATCA
- 5'...TGm6A TCA(N)5 TGCT...3'
Specifications
| 10 U/μL | |
| 55°C | |
| >95% of DNA fragments can be ligated and re-cut after 10-fold over-digestion with BclI | |
| Keep container tightly closed | |
| T.GATCA | |
| Liquid |
| 25 to 50% Water, >50% Glycerin | |
| 7.4 | |
| 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol | |
| 3000 U | |
| BcII |