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  5. 11924802

OPTIZYME™ BstEII (Eco91I), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8038-1

Additional Details : Weight : 0.01000kg

Product Code. 11924802

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...G^G T N A C C...3'
3'...C C A N T G^G...5'

Supplied with: 10X OPTIZYME Buffer 3
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 3: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl)2 , 100mM NaCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 20 - 50%
  • Buffer 3: 100%
  • Buffer 4: No reaction
  • Buffer 5: 50 - 100%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of BstEII is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Compatible Ends: G^GTSACC - MaeIII, NmuCI; G^GTWACC - MaeIII
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: May overlap - no effect
    • 5'...Cm5CW GGTNACm5CW GG...3'
  • 3'...G GWm5CCANTG GWm5CC...5'
  • (No cleavage effect)
  • CpG: May overlap - no effect
    • 5'...m5C GGTNACm5C G...3'
  • 3'... Gm5CCANTG Gm5C...5'
  • (No cleavage effect)
  • EcoKI: May overlap - effect not determined
  • EcoBI: May overlap - effect not determined

    • Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BstEII
    Keep container tightly closed
    G.GTNACC
    Liquid
    25 to 50% Water, >50% Glycerin
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
    2000 U
    BstEII
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