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OPTIZYME™ DraI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8026-1

Additional Details : Weight : 0.01000kg

Product Code. 11934792

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...T T T^A A A...3'
3'...A A A^T T T...5'

Supplied with: 10X OPTIZYME Buffer 4
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer 4: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 50 - 100%
  • Buffer 2: 50 - 100%
  • Buffer 3: 20 - 50%
  • Buffer 4: 100%
  • Buffer 5: 20 - 50%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton X-100, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with DraI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 5 units of DraI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Methylation Effects:
  • Dam: Never overlaps - no effect
  • Dcm: Never overlaps - no effect
  • CpG: Never overlaps - no effect
  • EcoKI: May overlap - blocked
    • 5'...TTTAAm6AC(N)6G TGC...3'
  • 3'...AAATT TG(N)6Cm6ACG...5'
  • (Cleavage is blocked)
  • EcoBI: Never overlaps - no effect
  • Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with DraI
    Keep container tightly closed
    TTT.AAA
    Liquid
    10X OPTIZYME™ Buffer 4
    7.4
    10mM Tris HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.15% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
    2000 U
    DraI
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