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  5. 11954792

OPTIZYME™ Sau3AI (Bsp143I), Fisher BioReagents™

Brand:  Fisher Bioreagents BP8030-1

Additional Details : Weight : 0.01000kg

Product Code. 11954792

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...^G A T C ...3'
3'... C T A G^...5'

Supplied with: 10X OPTIZYME Buffer Sau3AI
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer Sau3AI: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate, 0.02% Triton X-100 and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 20 - 50%
  • Buffer 2: 20 - 50%
  • Buffer 3: 0 - 20%
  • Buffer 4: 50 - 100%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.4 at 25°C), 10mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 95% of the DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI
  • Compatible Ends: BamHI, BclI, BglII, PsuI
  • Methylation Effects:
  • Dam: Completely overlaps - no effect
    • 5'...Gm6ATC...3'
  • (No effect on cleavage)
  • Dcm: Never overlaps - no effect
  • CpG: May overlap - blocked
    • 5'...GATm5C G...3'
  • 3'...CTA Gm5C...5'
  • (Cleavage is blocked)
  • EcoKI: Never overlaps - no effect
    • 5'...TGm6ATC(N)6 TGCT...3'
  • 3'...AC TAG(N)6 m6ACGA...5'
  • (No effect on cleavage)
  • EcoBI: May overlap - no effect

    • Specifications

    Specifications

    10 U/μL
    37°C
    >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Sau3AI
    300 U
    Sau3AI
    10X OPTIZYME™ Buffer Sau3AI
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    .GATC.
    Liquid
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