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  5. 11914802

OPTIZYME™ ScaI, Fisher BioReagents™

Brand:  Fisher Bioreagents BP8037-1

Additional Details : Weight : 0.01000kg

Product Code. 11914802

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Technical Support team on 056 260 260 for assistance.


Description

Description

5'...A G T^A C T...3'
3'...T C A^T G A...5'

Supplied with: 10X OPTIZYME AarI, ScaI, PasI Buffer
Conditions for 100% Activity:

  • 1X OPTIZYME Buffer AarI, ScaI, PasI: 10mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10mM MgCl2 , 100mM KCl and 0.1mg/ml BSA
  • Incubate at 37°C
Enzyme Activity in OPTIZYME buffers:
  • Buffer 1: 0 - 20%
  • Buffer 2: 0 - 20%
  • Buffer 3: 0 - 20%
  • Buffer 4: 0 - 20%
  • Buffer 5: 0 - 20%
Storage Buffer:
  • 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA and 50% (v/v) glycerol
  • Ligation and Re-cleavage:
  • More than 80% of the DNA fragments can be ligated and more than 90% of these can be re-cut after 50-fold over-digestion with ScaI.
  • Digestion of Agarose-embedded DNA:
  • A minimum of 20 units of ScaI is required for the complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours.
  • Notes:
    • Conditions of low salt, high enzyme concentration, glycerol concentration >5% or pH >8.0 may result in star activity.
    • Supercoiled plasmids may require up to 20-fold more ScaI for complete digestion than linear DNA.
    Methylation Effects:
    Dam: Never overlaps - no effect
    Dcm: Never overlaps - no effect
    CpG: Never overlaps - no effect
    EcoKI: Never overlaps - no effect
    EcoBI: May overlap - blocked
    • 5'...TGm6AGTACT(N)3 TGCT...3'
  • 3'...AC TCATGA(N)3 m6ACGA...5'
  • (Cleavage blocked)

    • Specifications

    Specifications

    10 U/μL
    37°C
    >80% of DNA fragments can be ligated and >90% of these can be re-cut after 50-fold over-digestion with ScaI
    1000 U
    ScaI
    10X OPTIZYME™ AarI, ScaI, PasI Buffer
    7.4
    10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
    AGT.ACT
    Liquid
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