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Invitrogen™ Human SMAD3 (Phospho) [pS423/pS425] InstantOne™ ELISA Kit

Product Code. 15530817
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Quantity:
96 Tests
Unit Size:
96 tests
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Product Code. 15530817

Brand: Invitrogen™ 858619211

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This item is not returnable. View return policy

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human SMAD3 (Ser423/425) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate. Suitable Sample Types Tested on cell culture samples. Expected to work on many other sample types, but have not been tested at this time.

SMAD3 belongs to the SMAD family of proteins. They are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P84022
Conjugate HRP
Product Type ELISA
Sample Type Cell Lysates
Target Species Human
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4088
Gene Symbol HSPC193,HsT17436,JV15-2,LDS1C,LDS3,MADH3
Kit Contents Capture Antibody, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Cell Lysate 20 μL
Storage Requirements 2°C to 8°C
Synonym Mothers against decapentaplegic homolog 3
Target SMAD3
Test Time 1 hrs 20 mins
Total Assay Time 1 hrs 10 mins
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