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Molecular Probes™ pHrodo™ Green Maleimide

pHrodo™ Green Maleimide

Brand:  Molecular Probes™ P35370

Additional Details : Weight : 0.01000kg

Product Code. 13345654

  • 672.00€ / 1mg
Estimated Shipment: 08-04-2024
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Description

Description

The thiol-reactive pH-sensitive pHrodo™ Green Maleimide dye is suitable for the creation of bioconjugates to study endocytosis and phagocytosis. pHrodo™ Green dramatically increases fluorescence as the pH of its surroundings become more acidic.
  • Use pH-sensitive pHrodo™ Green Maleimide to make pH-sensitive bioconjugates of your choice
  • Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
  • Multiplex with red fluorescent markers such as RFP, pHrodo™ Red, and many others

    The increase in fluorescence of pHrodo™ Green as pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it an ideal tool to study endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo™ Green makes it useful for multi-color experiments. pHrodo™ Green can be detected using most standard green fluorescent filter sets and has been validated for use on a variety of platforms including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo™ Green in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.

    pHrodo™ Green Maleimide is a thiol-reactive dye that can be used to create pHrodo™ Green bioconjugates in aqueous buffer. The maleimide reacts with free sulphydryl groups produced by the reduction of cysteines in proteins or peptides. Maleimides are particularly useful for labeling antibodies as the dye will not attach to the antibody binding site. This reaction will result in a stable conjugate that can be used in live cell assays or stored for later use.

    pHrodo™ Green is also available in an amine-reactive form (see pHrodo™ Green STP), as well as a selection of ready-to-use conjugates (e.g., E. coli, S. aureus, and dextran). In addition, pHrodo™ Red reactive dyes and ready-to-use conjugates are available as a color alternative with the same properties.

    For Research Use Only. Not for human or animal therapeutic or diagnostic use.
  • Specifications

    Specifications

    Dried Down
    Maleimide
    1 vial , store ≤ -20 °C, dessicate and protected from light.
    Fluorescent
    Thiol
    Green
    525
    1 mg
    DMSO (Dimethylsulfoxide)
    Thiol
    75000
    pHrodo™ Green
    pHrodo™
    505
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    For Research Use Only. Not for use in diagnostic procedures.