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Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

95.00€ - 384.00€

Specifications

GC-Rich PCR Performance High
Polymerase Phusion Flash II DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity PCR Master Mix
Shipping Condition Dry Ice
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10043967
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Thermo Scientific™
F548S
100 Reactions
95.00€
100 reactions
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10659009
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Thermo Scientific™
F548L
500 Reactions
384.00€
500 reactions
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Description

Description

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to add template and primers minimizing the number of pipetting steps.

The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features
• Extreme speed: extension times of 15 s/kb or less
• Hot start modification allowing 'zero-time reactivation'
• Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq
• High yields in reduced time

Applications
• Fast PCR
• High-fidelity PCR
• Difficult (GC-rich) templates
• Template generation for sequencing
• Multiplex PCR
• Long-range PCR
• Cloning
• Mutagenesis
• Microarray

Using Phusion DNA Polymerases
Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our Tm calculator at www.thermofisher.com/tmcalculator.

Specifications

Specifications

High
Fast
Dry Ice
2X
Built-In Hot Start
SuperMix or Master Mix
Phusion Flash II DNA Polymerase
High-Fidelity PCR Master Mix
Hot-start PCR, High-fidelity PCR
25X
Blunt
20 kb or less
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.