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Thermo Scientific™ Pierce™ CDI-activated Agarose Resin

Product Code. 10239963 Shop All Thermo Scientific Products
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10 mL
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10mL
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Product Code. 10239963 Supplier Thermo Scientific™ Supplier No. 20259
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Description

Covalently immobilize N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions using carbonyldiimidazole-activated resin.

Thermo Scientific™ Pierce CDI-Activated Agarose is a porous, beaded chromatography support that is activated with carbonyldiimidazole groups for covalent immobilization of amine-containing affinity ligands.

Pierce CDI-activated Agarose is a porous, beaded chromatography support that is activated with carbonyl diimidazole groups for covalent immobilization of proteins and other molecules that contain N-nucleophiles. Coupling can be performed in aqueous or organic solvent conditions, making possible the immobilization of many kinds of ligands. Once a molecule is immobilized, the prepared affinity resin can be used multiple times in typical affinity purification procedures.

Highlights:

  • Reliable coupling chemistry – immobilization occurs through the reaction of N-nucleophiles with 1,1'-carbonyldiimidazole groups of the resin to form stable, uncharged N-alkylcarbamate linkages

  • Easy to use – no secondary reagents needed; just wash equilibrate resin in alkaline coupling buffer and add ligand; reaction proceeds spontaneously

  • Stable activation – half-life of hydrolysis is much longer than hydroxysuccinimide ester activations, making immobilization reactions easier to prepare and control; simplifies filtration and washing before adding a ligand or protein

  • Well-defined coupling condition – reaction is most efficient with primary amines at pH 9 to 11

Properties of CDI-Activated Agarose:

  • Resin: crosslinked 6% beaded agarose
  • Activation: at least 50μmol 1,1'-carbonyldiimidazole (CDI) groups per milliliter of resin
  • Format: supplied as stabilized, 50% slurry in acetone
TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at 4°C.
Product Line Pierce
Quantity 10 mL
Target Primary Amine
Type Resin
Stationary Phase Carbonyl Diimidazole
Format Bottle
Column Type Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.

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