Thermo Scientific™ Pierce™ Protein A/G Coated Plate IP Kit

Highlights:

• Ready-to-use, high quality coated plates provide high capacity and consistency
• Accommodate antibody and sample volumes comparable to tube-based methods
• Easily recover immunoprecipitated samples for downstream analysis
• Coated plates are 96-well strip plates, convenient for experiments requiring only a partial plate
• Include simple instructions, including detailed explanation of appropriate controls
• Two kits available, suitable for most common antibody types (mouse, rabbit, human and goat IgG subclasses) or any biotinylated antibody or bait protein

Advantages of the Plate Format:

• Best suited for simultaneously processing multiple samples and their control conditions
• Allows faster, easier and more thorough washing than with traditional tube/resin IP methods
• Makes use of familiar and convenient ELISA tools (multichannel pipettors and plate washing)
• No no tubes to open and close, and no centrifugation required

Protein A/G Kit (Part No. 45350)

• Binds all human IgG subclasses; human IgM, IgA (weak), and IgE; and all mouse IgG subclasses
• Offers increased binding for mouse monoclonal antibodies compared to Protein A or Protein G alone
• Free of pH dependency when binding IgGs, providing more flexible binding conditions

Streptavidin Kit (Part No. 45360)

• Used in combination with biotinylated antibodies, resulting in IP and co-IP preparations free of interfering antibody fragments
• Contains biotin-blocking buffer to minimize endogenous biotin binding

Includes:

Coated 96-well strip plates, uncoated 96-well strip plates, PBS pack, detergent solution, elution buffer, neutralization buffer and sealing tape

Recommended for:

Rapid, trouble-free immunoprecipitation of multiple samples and their controls; Immunoprecipitation when plate-based tools and techniques are preferred