anti-NeutrAvidin, Thermo Scientific™
NeutrAvidin Protein, 31000, from Invitrogen™, 31000, from Invitrogen™ Species Reactivity: ; Applications: ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot.
Marque: Thermo Scientific Pierce 31000
Informations supplémentaires : Poids : 1.81440kg
DescriptionNeutrAvidin Protein is deglycosylated native avidin from egg whites. Removal of the excess carbohydrate by an exclusive process yields a protein with a more neutral isoelectric point and less nonspecific binding properties. Purified and conjugated forms of NeutrAvidin Protein provide exceptional performance in Western blot, ELISA and IHC applications that require biotin-binding probes. Assay specificity, sensitivity, and signal-to-noise ratios with NeutrAvidin Protein are generally equivalent or better than with the significantly more expensive streptavidin. Avidin is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibia. The biotin-binding protein contains four identical subunits having a combined mass of 67,000-68,000 daltons. Removing the glycosylation from avidin yields NeutrAvidin Protein with a mass of 60,000 daltons. Carbohydrate-based lectin-binding is reduced to undetectable levels, yet biotin-binding affinity is retained. NeutrAvidin Protein offers the advantages of a neutral pI to minimize nonspecific adsorption, along with lysine residues that remain available for derivatization or other customized conjugation. NeutrAvidin Protein yields the lowest nonspecific binding among the known biotin binding proteins. The specific activity for biotin-binding is approximately 14µg/mg of protein, which is near the theoretical maximum activity.
Formulation: 10mg purified protein and no preservative
31000 contains 10 mg of lyophilized powder from modified avidin in salt-free solution.
|ELISA, Immunohistochemistry, In Situ Hybridization (ISH), Western Blot|
|Poudre lyophilisée à partir d’avidine modifiée dans une solution sans sel|