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Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)

Product Code. 11812164 Shop All Thermo Scientific Products
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Quantity:
100 mg
Unit Size:
100mg
Product Code. Quantity unitSize
11812164 100 mg 100mg
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Product Code. 11812164

Brand: Thermo Scientific™ 26129

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Description

Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce PEG12-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 12-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

SPDP reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithio group at the opposite end. PEG12-SPDP has a 12-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.

Highlights:

  • Efficient amine conjugation – NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines

  • Reversible sulfhydryl conjugation – pyridyldithio group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond

  • Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules

  • Spacer arm – connects primary amines and sulfhydryl targets at distances up to 54.1 angstroms

Specifications:

  • Chemical name (simplified): 2-pyridyldithiol-tetraoxaoctatriacontane-N-hydroxysuccinimide
  • Chemical formula: C39H65N3O17S2
  • Molecular weight: 912.07
  • Spacer arm: 54.1 angstroms
  • Form: light, viscous liquid
  • Solubility: dissolve 2mg in 110μL DMSO or DMF to make 20mM stock for each use
TRUSTED_SUSTAINABILITY

Specifications

Chemical Reactivity Amine-Sulfhydryl
Cleavable By Thiols
Description PEG12-SPDP
Molecular Weight (g/mol) 912.07
PEGylated Yes
Spacer Arm Length 54.1 Å
Content And Storage Upon receipt store at -20°C protected from moisture.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Pyridyldisulfide
Spacer Long (>30 Å)
Form Liquid
Quantity 100 mg
Solubility Water
Format Standard
Water Soluble Yes
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.

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