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Tocris Bioscience™ preQ1-biotin
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Product Code. 18758986
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Quantity:
5 mg
Unit Size:
5mg
Product Code. Quantity unitSize
18758986 5 mg 5mg
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Product Code. 18758986

Brand: Tocris Bioscience™ 7804/5

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337.40 EUR valid until 2026-03-31
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Description

PreQ1 with biotin handle for RNA labeling and purification

preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.

Plasmids for expression of the E.coli TGT enzyme ( #138201 ) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end ( #138209 ) are available from Addgene.

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Specifications

Chemical Name or Material N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
Quantity 5 mg
Target Buffers, Chelators and Reagents
Molecular Formula C23H36N8O3S
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