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  5. Invitrogen™ IL-17A Rat Uncoated ELISA Kit with Plates

Invitrogen™ IL-17A Rat Uncoated ELISA Kit with Plates

Product Code. p-7091925
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Quantity:
10 x 96 Tests
2 x 96 Tests
Unit Size:
Each
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Contains Azide

Product Code. 12770160

Brand: Invitrogen™ 88717022

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This item is not returnable. View return policy
Contains Azide

Description

Includes

  • Capture Antibody: Pre-titrated, purified antibody
  • Detection Antibody. Pre-titrated, biotin-conjugated antibody
  • Standard. Recombinant cytokine for generating standard curve and calibrating samples
  • ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
  • Assay Diluent. 5X concentrated
  • Detection enzyme. Pre-titrated Avidin-HRP
  • Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution

Specifically engineered for accurate and precise measurement of rat IL-17A homodimer protein levels from samples including serum and cell culture supernatants.

The Rat Interleukin 17A (IL-17A) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of rat IL-17A. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
TRUSTED_SUSTAINABILITY

Specifications

Accession Number Q61453
Assay Range 2-200 pg/mL
Assay Sensitivity 2 pg/mL
Conjugate Biotin
Product Type ELISA Kit
Sample Type Serum, Supernatant
Target Species Rat
For Use With (Application) ELISA
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 301289
Gene Symbol DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
Kit Contents Capture Antibody: Pre-titrated; purified antibody, Detection Antibody: Pre-titrated; biotin-conjugated antibody, Standard: Recombinant cytokine for generating standard curve and calibrating samples, ELISA/ELISPOT Coating Buffer Powder: This uncoated ELISA kit with plates may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 μM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20)., Assay Diluent: 5X concentrated, Detection enzyme: Pre-titrated Avidin-HRP, Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution, Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards, 96 Well Plate: Corning Costar 9018 (included with product Cat. Nos. ending in suffixes -22; -44; -76; -86)
Label or Dye HRP
Quantity 2 x 96 Tests
Regulatory Status RUO
Sample Volume Serum, 100 μL; Supernatant, 100 μL
Storage Requirements 2°C to 8°C
Synonym Interleukin-17A, IL-17AA
Target Rat
Test Time 1 hr. 45 min.
Total Assay Time 24 hr. 30 min.
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For Research Use Only

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